Identification of the serotype-specific and group-specific antigens of bluetongue virus.

Serotype Identification of Recent Iranian Isolates of Infectious Bronchitis Virus by Type-Specific Multiplex RT-PCR

Detection of specific antigens of Newcastle disease virus using an absorbed Western blotting method

Newcastle disease virus (NDV) is an economically important poultry pathogen with a worldwide distribution that may infect a wide range of domestic and wild avian species. The identification of different pathotypes of NDVs plays an important role in the diagnosis and development of vaccines to control and eradicate NDV infections. In our previous study, we showed that mono-specific antibodies ca...

Serological survey of sheep in Iran for Type specific Antibody to Bluetongue virus

Isolation and pathogenicity identification of avian paramyxovirus serotype 1 (Newcastle disease) virus from a Japanese quail flock in Iran

Newcastle disease virus (NDV) was isolated and identified for the first time from a flock of Japanese quail suspected to ND in Iran. Birds of the flock aged variously, and some of them died from the disease. Loss of appetite, weakness and decrease in egg production, diarrhea and nervous symptoms were the main clinical signs in the birds. Hemorrhagic lesions of the intestinal tracts and proventr...

Serological differentiation of five bluetongue virus serotypes in indirect ELISA.

The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific f...